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[Cloning and expression analyses of down-regulated cDNA C6-2A in human esophageal cancer]
1National Laboratory of Molecular Oncology, Department of Cell Biology, Cancer Institute, Chinese Academy of Medical Sciences and Peking Union Medical college, Beijing 100021 P.R.China. wangmr@pubem.cicams.ac.cn
Objective:
To clone genes associated with the genesis of human esophageal cancer.
Methods:
Identifying missing or low expressing cDNAs in human esophageal cancer tissues by mRNA differential display and examining its mRNA expression in 4 human cancer cell lines, 9 fetal tissues and other matched esophageal cancer tissues by Northern blot, dot blot and RT-PCR.
Results:
One cDNA fragment named C6-2A, was cloned and sequenced. There was no identical sequence with C6-2A in BLASTN database; but in querying Genbank EST, the authors found that C6-2A was identical with ne27b03.s1NCI-CGAP-C03 humans sapiens cDNA clone IMAGE:898541 3' and zv30g07.rl Soares ovary tumor NbHOT homo sapiens cDNA clone 755196. 6/6 esophageal cancer tissues in Northern blot and 7/8 in dot blot did not or slightly express C6-2A. RT-PCR analysis showed that C6-2A was expressed much lower in 17/20 esophageal cancer tissues than adjacent microscopically normal mucosa, highly expressed in fetal esophageal mucosa, skin, cerebrum, placenta; moderately expressed in fetal stomach and liver, but not detected in fetal heart, small intestine and kidney.
Conclusion:
The high frequency of deletion of decreased expression of C6-2A in esophageal cell lines and human esophageal cancer tissues suggested that C6-2A might be involved in the carcinogenesis of esophagus.
Insights
A novel gene, C6-2A, was identified and found to be significantly downregulated in human esophageal cancer tissues and cell lines. This suggests C6-2A may play a crucial role in esophageal carcinogenesis.
Area of Science:
- Molecular oncology
- Cancer genomics
- Gene expression profiling
Context:
- Esophageal cancer is a significant global health concern with complex genetic underpinnings.
- Identifying novel genes involved in carcinogenesis is crucial for understanding disease development.
- Previous research has focused on known oncogenes and tumor suppressors, leaving potential novel candidates unexplored.
Purpose:
- To clone and characterize genes associated with the development of human esophageal cancer.
- To investigate the expression patterns of a newly identified cDNA fragment, C6-2A, in esophageal cancer.
- To determine the potential role of C6-2A in esophageal carcinogenesis.
Summary:
- A cDNA fragment, designated C6-2A, was cloned and sequenced. Its sequence showed no identity in BLASTN but was found in GenBank ESTs.
- Northern blot and dot blot analyses revealed absent or low expression of C6-2A in most esophageal cancer tissues.
- RT-PCR demonstrated significantly lower C6-2A expression in 17/20 esophageal tumors compared to adjacent normal tissue, with high expression in fetal tissues and placenta.
Impact:
- The frequent deletion or decreased expression of C6-2A in esophageal cancer cell lines and tissues suggests its involvement in esophageal carcinogenesis.
- C6-2A represents a potential novel biomarker or therapeutic target for esophageal cancer.
- Further research into C6-2A's function could elucidate key mechanisms in esophageal cancer development.