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M1/MUC5AC mucin released by human airways in vitro
1INSERM U 482, Hôpital Saint-Antoine, Paris, France.
The European Respiratory Journal
|October 9, 1999
Summary
Monoclonal antibodies detected M1 mucin (MUC5AC) in human airways. This M1/MUC5AC mucin is primarily released from epithelial goblet cells and its secretion is constant, not significantly altered by secretagogues.
Area of Science:
- Respiratory Medicine
- Biochemistry
- Immunohistochemistry
Background:
- Monoclonal antibodies (anti-M1 MAbs) recognize M1 mucin, the product of the human MUC5AC gene.
- M1 mucin plays a role in airway secretions and epithelial protection.
Purpose of the Study:
- To quantify M1 mucin in human bronchial epithelium and airway fluid.
- To investigate the source and regulation of M1 mucin secretion.
Main Methods:
- Immunohistochemistry was used to detect M1 mucin in bronchial tissue sections.
- An immunoradiometric assay measured M1 mucin concentration in bronchial fluid.
- Human bronchial preparations were stimulated with methacholine, leukotriene D4, or anti-immunoglobulin E.
Main Results:
- Anti-M1 MAbs intensely stained goblet cells in the bronchial epithelium.
- M1/MUC5AC mucin levels in secreted fluids remained stable despite secretagogue stimulation.
- Epithelial removal significantly reduced M1/MUC5AC mucin detection in bronchial fluids.
Conclusions:
- M1/MUC5AC mucin is primarily released from goblet cells within the epithelial layer of human airways.
- Airway M1/MUC5AC mucin secretion appears to be a continuous process.
- The study provides insights into the origin and continuous release of M1 mucin in respiratory secretions.