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Regulation of mouse somatostatin receptor type 2 gene expression by glucocorticoids
1Department of Pharmacology and Toxicology, University of Magdeburg, Leipzigerstrasse 44, D-39120, Magdeburg, Germany. Juergen.Kraus@medizin.uni-magdeburg.de
Abstract:
Somatostatin is a regulatory peptide with important inhibitory functions. Its effects are mediated by five receptors, sst1-sst5. Previous studies revealed that sst2 contains three alternative, tissue specific promoters. Here, molecular mechanisms for the regulation of sst2 transcription by glucocorticoids were investigated. Reporter gene assays in NG 108-15 cells and electrophoretic mobility shift assays revealed that a glucocorticoid responsive element at position -1044 on the second promoter mediates dexamethasone induction. These findings, the existence of a cAMP response element and the tissue dependent activity (brain, pituitary and gastrointestinal tissues) indicate the importance of the second sst2 gene promoter.