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Key adhesion molecules are present on long podia extended by hematopoietic cells
W Holloway1, A R Martinez, D J Oh
1Department of Bioengineering, University of California at San Diego, La Jolla, California.
Cytometry
|October 16, 1999
Summary
Scientists identified adhesion molecules on cell projections called podia. This technique reveals their role in cell migration and communication, advancing our understanding of hematopoietic cells.
Area of Science:
- Cell Biology
- Hematology
- Immunology
Background:
- CD34(+) hematopoietic cells and KG1a cells form long, thin podia that extend and retract dynamically.
- These podia can adhere to substrates and connect cells up to 300 microm apart.
- The surface receptors on these podia have not been previously described.
Purpose of the Study:
- To describe a method for detecting surface receptors on cell podia.
- To identify specific adhesion molecules present on these podia.
- To investigate the role of podia in cell migration and communication.
Main Methods:
- Utilized time-lapse fluorescent microscopy and immunostaining techniques.
- Developed an in situ antibody staining procedure without cell fixation.
- Examined CD34 selected mobilized peripheral blood cells and KG1a cells.
Main Results:
- Demonstrated the presence of key adhesion molecules (CD11a, CD18, CD29, CD34, CD45, CD49d, CD49e, CD62L) on cell podia.
- Identified CD54 and CD44 on KG1a cell podia, but not on primary CD34(+) cells.
- Observed that KG1a and CEM cell lines, unlike AML-193 and MV4-11, extend long podia with similar morphology and adhesion molecule expression.
Conclusions:
- Developed a novel technique for detecting surface receptors on thin cell membrane projections (podia).
- Results indicate that these podia likely play a significant role in cell migration.
- Podia are also suggested to be involved in intercellular communication processes.