Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

White Blood Cell Enumeration and Differential by Flow Cytometry: The ICSH WBC Reference Method.

International journal of laboratory hematology·2025
Same author

Phase 1/2 trial of anti-CD7 allogeneic WU-CART-007 for patients with relapsed/refractory T-cell malignancies.

Blood·2025
Same author

Fifth edition WHO classification: precursor lymphoid neoplasms, acute leukaemias of mixed or ambiguous lineage, myeloid/lymphoid neoplasms, and histiocytic and dendritic cell neoplasms, including strategies for application in resource-limited settings.

Journal of clinical pathology·2025
Same author

Fifth edition WHO classification: myeloid neoplasms.

Journal of clinical pathology·2025
Same author

The Proteomics of T-Cell and Early T-Cell Precursor (ETP) Acute Lymphocytic Leukemia: Prognostic Patterns in Adult and Pediatric-ETP ALL.

Cancers·2025
Same author

Reply to: Accurate Determinants of Outcome in ALL.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2024

Related Experiment Video

Updated: Jun 29, 2026

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
11:54

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues

Published on: October 20, 2019

Multicenter clinical experience with flow cytometric method for fetomaternal hemorrhage detection.

Jenn C Chen1, Bruce H Davis, Brent Wood

  • 1Department of Clinical Pathology, William Beaumont Hospital, Royal Oak, Michigan, USA.

Cytometry
|December 24, 2002
PubMed
Summary

Flow cytometric method (FCM) offers a superior alternative to the Kleihauer-Betke (KB) test for detecting fetal red blood cells in maternal blood, crucial for managing fetomaternal hemorrhage (FMH). This advanced technique provides more objective and precise results, aiding in timely clinical decisions.

More Related Videos

Human Fetal Blood Flow Quantification with Magnetic Resonance Imaging and Motion Compensation
06:56

Human Fetal Blood Flow Quantification with Magnetic Resonance Imaging and Motion Compensation

Published on: January 7, 2021

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
08:48

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

Related Experiment Videos

Last Updated: Jun 29, 2026

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
11:54

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues

Published on: October 20, 2019

Human Fetal Blood Flow Quantification with Magnetic Resonance Imaging and Motion Compensation
06:56

Human Fetal Blood Flow Quantification with Magnetic Resonance Imaging and Motion Compensation

Published on: January 7, 2021

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
08:48

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

Area of Science:

  • Hematology
  • Immunology
  • Clinical Diagnostics

Background:

  • Accurate enumeration of fetal red blood cells (RBCs) is critical for managing fetomaternal hemorrhage (FMH), especially in Rh-incompatible pregnancies.
  • Fetomaternal hemorrhage (FMH) can lead to significant fetal complications, necessitating reliable diagnostic methods.

Purpose of the Study:

  • To evaluate the efficacy and superiority of the flow cytometric method (FCM) using an anti-hemoglobin F (HbF) antibody compared to the manual Kleihauer-Betke (KB) test for detecting fetal RBCs in maternal circulation.
  • To assess the clinical utility and transportability of the FCM method across multiple institutions.

Main Methods:

  • Multi-institutional evaluation of the flow cytometric method (FCM) for fetal RBC detection using anti-hemoglobin F (HbF) monoclonal antibody.
  • Comparison of FCM results with the manual Kleihauer-Betke (KB) test, including analysis of fluorescence intensity and forward light scatter.
  • Assessment of test transportability through inter-laboratory comparisons and review of College of American Pathologists survey data.

Main Results:

  • During 1997-2001, 5.5% of patients had detectable fetal RBCs, with 1.7% having >30 mL of fetal blood (large FMH).
  • A high mortality rate of 64% was observed in infants with large FMH.
  • FCM demonstrated significant differences in anti-HbF staining intensity and forward light scatter between fetal and adult RBCs, confirming its specificity and sensitivity. FCM proved superior to the KB test.

Conclusions:

  • Flow cytometric method (FCM) is a simpler, more objective, and precise alternative to the Kleihauer-Betke (KB) method for clinical detection of fetomaternal hemorrhage (FMH).
  • The high mortality associated with large FMH underscores the need for laboratories to adopt the more robust FCM method over the KB test for improved patient outcomes.