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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jan 18, 2026

Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro

Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro

Published on: December 26, 2014

White Blood Cell Enumeration and Differential by Flow Cytometry: The ICSH WBC Reference Method.

Benjamin D Hedley¹, Michael Keeney¹, Peter Gambell²

¹London Health Science Center, London, Ontario, Canada.

International Journal of Laboratory Hematology

|September 12, 2025

Summary

This summary is machine-generated.

A new flow cytometry method offers an improved reference for white blood cell (WBC) differential counts, outperforming manual methods for rare cell populations and correlating well with automated analyzers.

Keywords:

Immuno‐differential analyzer flow cytometry hematology reference

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Related Experiment Videos

Last Updated: Jan 18, 2026

Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro

Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro

Published on: December 26, 2014

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

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Published on: October 17, 2018

Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples

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Published on: July 6, 2014

Area of Science:

Hematology
Clinical Pathology
Flow Cytometry

Background:

Manual white blood cell (WBC) differential counts are the current standard but face challenges.
These include interpreter expertise, staining quality, and imprecision with low cell counts.

Purpose of the Study:

To describe and validate an alternative, single-tube, 8-color flow cytometric reference method for WBC differential analysis.
To compare its performance against the manual reference method and current hematology analyzers.

Main Methods:

An international comparison involving 120 normal and 496 abnormal clinical samples was conducted.
Four institutions utilized four different flow cytometer models to assess the proposed method.

Main Results:

The flow cytometric method demonstrated equivalent performance to the manual smear review for WBC differential counts.
It showed improved performance for identifying low/infrequent cell populations like monocytes and basophils.
Good correlation was observed with automated hematology analyzers for total WBC enumeration.

Conclusions:

The flow cytometric method, termed "ICSH WBC reference," can serve as a reliable alternative to CLSI H20-A2 for WBC enumeration and differential counting.
This method is particularly valuable for evaluating automated differential counters and improving accuracy for rare cell types.