Related Experiment Video
Updated: Jun 19, 2026
Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
Published on: March 24, 2023
Flow cytometric quantitation of CD34+ progenitors in peripheral blood: an adjunct tool for diagnostic evaluation of
Lauren Reiman1, My H Vu2, Michael Cortez-Bacolot3
1Keck School of Medicine, University of Southern California, Los Angeles, CA, United States.
Objectives:
In healthy individuals, CD34+ stem cells are detectable in peripheral blood in relatively low and stable numbers. The clinical utility of peripheral blood CD34+ stem cell quantitation in the evaluation of pancytopenia has not been systematically studied.
Methods:
We used a large archival clinical cohort (n = 154) of pediatric patients from our pediatric-only institution without a history of hematologic disorder or circulating blasts on morphology who underwent peripheral blood flow cytometric screening for any reason. Circulating CD34+ stem cells and total progenitors were quantified and correlated with clinical characteristics and outcomes.
Results:
Patients ultimately diagnosed with aplastic anemia (n = 15) showed significantly decreased circulating progenitors compared with those with cytopenias of alternative causes, whereas patients with infectious, inflammatory, or other presumed reactive conditions often demonstrated larger CD34+ progenitor populations, consistent with preserved or responsive marrow function. Using receiver operating characteristic analysis, the optimal cutoff for %CD34+ progenitors was 0.0124%, which was rounded to 0.01% for clinical interpretability.
Conclusions:
These findings support the potential clinical utility of peripheral blood CD34+ quantitation in the diagnostic workup of pediatric pancytopenia.
