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A chemiluminescent catecholamine assay: its application for monitoring adrenergic transmitter release
1Laboratoire de Neurobiologie, Cellulaire et Moléculaire, C.N.R.S., Gif-Sur-Yvette, France.
Journal of Neuroscience Methods
|October 16, 1999
Abstract:
A chemiluminescent procedure for measuring catecholamines (dopamine, norepinephrine, epinephrine) is described. It is based on the observation that lactoperoxidase catalyses both the oxidation of catecholamines, and the chemiluminescent reaction of luminol with their oxidation product. The assay has been adapted for continuously monitoring the release of catecholamines from adrenergic tissues, from cell suspensions and from cells loaded in culture with dopamine.