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Related Experiment Videos

Vg1 RBP intracellular distribution and evolutionarily conserved expression at multiple stages during development.

Q Zhang1, K Yaniv, F Oberman

  • 1Department of Anatomy and Cell Biology, Hebrew University, Hadassah Medical School, Jerusalem, Israel.

Mechanisms of Development
|October 19, 1999
PubMed
Summary
This summary is machine-generated.

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Vg1 RBP protein and RNA show distinct localization patterns during Xenopus oogenesis and embryogenesis. This expression is conserved in zebrafish, indicating a crucial role in early development.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Vg1 RBP is involved in localizing Vg1 mRNA to the vegetal cortex in Xenopus oocytes.
  • Understanding Vg1 RBP's expression is key to deciphering early developmental mechanisms.

Purpose of the Study:

  • To analyze the expression and intracellular distribution of Vg1 RBP during oogenesis and embryogenesis in Xenopus.
  • To investigate the conserved role of Vg1 RBP by examining its expression in zebrafish.

Main Methods:

  • In situ hybridization was used to analyze Vg1 RBP RNA localization in Xenopus and zebrafish embryos.
  • Cloning of the zebrafish Vg1 RBP homolog was performed using EST screening.

Main Results:

  • Vg1 RBP protein colocalizes with Vg1 RNA in Xenopus oocytes.

Related Experiment Videos

  • Vg1 RBP RNA localizes to the animal pole in oocytes but shows widespread zygotic expression in neural tissues and derivatives in Xenopus.
  • Zebrafish Vg1 RBP RNA exhibits similar animal pole localization in oocytes and conserved zygotic expression patterns compared to Xenopus.

    • Conclusions:

    • Vg1 RBP exhibits distinct maternal and zygotic expression patterns.
    • The zygotic expression of Vg1 RBP is conserved between Xenopus and zebrafish, highlighting its importance in vertebrate development.