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Published on: February 10, 2022
Vaccine entrapment in liposomes
G Gregoriadis1, B McCormack, M Obrenovic
1The School of Pharmacy, University of London, 29-39 Brunswick Square, London, WC1N 1AX, United Kingdom. gregoriadis@cua.ulsop.ac.uk
Methods (San Diego, Calif.)
|October 20, 1999
Summary
This study presents a scalable liposome technology for high-yield vaccine entrapment. The dehydration-rehydration method effectively incorporates peptide, protein, DNA, and particulate vaccines into liposomes for enhanced delivery.
Area of Science:
- Biotechnology
- Vaccine Development
- Nanotechnology
Background:
- Liposomes are crucial for delivering peptide, protein, and DNA vaccines.
- Existing methods for vaccine entrapment in liposomes often lack scalability and efficiency.
- There is a need for simple, high-yield technologies for liposomal vaccine formulation.
Purpose of the Study:
- To develop and characterize a scalable liposome technology for high-yield entrapment of diverse vaccine types.
- To optimize the dehydration-rehydration procedure for efficient incorporation of soluble and particulate antigens.
- To evaluate the size reduction and antigen association of liposomes post-formulation.
Main Methods:
- Development of liposome generation techniques for various sizes and antigen types.
- Utilizing the dehydration-rehydration procedure involving freeze-drying and rehydration of vesicles and antigens.
- Employing microfluidization to reduce liposome size and sucrose gradient fractionation for particulate antigen association.
Main Results:
- The dehydration-rehydration procedure achieved over 90% entrapment of soluble vaccines (peptide, protein, DNA).
- Microfluidization reduced liposome size to approximately 100 nm while maintaining high antigen association.
- Up to 30% entrapment of particulate antigens (e.g., Bacillus subtilis spores) was achieved using giant liposomes.
Conclusions:
- The developed liposome technology is simple, scalable, and achieves high-yield entrapment for various vaccine types.
- This method offers a promising approach for formulating liposomal vaccines with improved delivery characteristics.
- The technology demonstrates versatility in encapsulating both soluble and particulate antigens for potential vaccine applications.
