Biophysical properties of mycobacteriophages

Biophysical characterization of two mycobacteriophages (Phlei phage and Butyricum phage) was carried out. Biophysical parameters obtained were: (i) buoyant densities in CsCl of I . 51 g/ml for both phages; (ii) sedimentation coefficient of 490 S and 410 S; (iii) DNA content of 42 and 34% and (iv) mol. wt. calculated by electron microscopic dimensions to be 123 x 10(6) and 116 x 10(6) for Butyricum and Phlei phage, respectively.