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A solid phase reverse transcriptase micro-assay for the detection of human immunodeficiency virus and other
P A Somogyi1, A Gyuris, I Földes
1National Institute of Hygiene, Microbiological Research Group, Budapest.
Abstract:
A simple and rapid solid-phase reverse transcriptase assay was developed based on the use of poly(rA):oligo(dT)12-18 as template primer immobilized on DEAE cellulose paper squares to detect human immunodeficiency virus (HIV) and/or other retroviruses in cell culture supernatants. It was found that PEG (per se) -up to 4% concentrations (w/v)--did not inhibit reverse transcriptase activity. Optimal conditions of the assay were determined. This solid-phase technique is much faster and more convenient than the methods described previously.
