Related Experiment Video

Updated: Aug 10, 2026

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
09:34

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species

Published on: November 8, 2016

Ribotyping of Vibrio parahaemolyticus isolates obtained from food poisoning outbreaks in Taiwan

H C Wong1, C Y Ho, L P Kuo

  • 1Department of Microbiology, Soochow University, Taipei, Taiwan, Republic of China. wonghc@mail.scu.edu.tw

Microbiology and Immunology
|October 21, 1999
PubMed

Insights

A new ribotyping method effectively characterizes Vibrio parahaemolyticus, a common foodborne pathogen. This technique identified 30 distinct ribotypes among 121 isolates, aiding molecular epidemiology.

Area of Science:

  • Microbiology
  • Molecular Epidemiology
  • Food Safety

Background:

  • Vibrio parahaemolyticus is a significant foodborne pathogen in Asia.
  • Accurate molecular typing is crucial for tracking outbreaks and understanding transmission.

Purpose of the Study:

  • To develop and evaluate a novel ribotyping method for Vibrio parahaemolyticus.
  • To assess the molecular epidemiology of Vibrio parahaemolyticus isolates from Taiwan.

Main Methods:

  • Genomic DNA fragmentation using HindIII digestion.
  • Hybridization with a cDNA probe for Escherichia coli 16S and 23S RNA genes.
  • Hierarchical cluster analysis of restriction fragment patterns.

Main Results:

  • The method differentiated 121 isolates into 30 distinct ribotypes.
  • Major ribotypes (A3, A7, E3, F1) and groups (A, E, F, G, B) were identified.
  • High discriminatory ability (Simpson's index of diversity = 0.93) was achieved.

Conclusions:

  • The developed ribotyping method is a powerful tool for molecular epidemiological studies of Vibrio parahaemolyticus.
  • This method offers comparable discriminatory power to pulsed-field gel electrophoresis.
  • It provides valuable insights into the genetic diversity of this foodborne pathogen.

More Related Videos

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates07:58

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

Published on: May 30, 2017

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

Related Concept Videos

Bacterial Gastroenteritis01:18

Bacterial Gastroenteritis

Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid receptor...
Cholera01:25

Cholera

Cholera is an acute gastrointestinal disease caused by the Gram-negative bacterium Vibrio cholerae. It is transmitted primarily via the fecal-oral route through the ingestion of contaminated water or food.Vibrio cholerae is a motile, Gram-negative bacterium of the family Vibrionaceae, primarily associated with waterborne outbreaks in areas with inadequate sanitation. Although over 200 serogroups of V. cholerae exist, only O1 and O139 are responsible for epidemic cholera. The O1 serogroup,...