Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cashing in on crystals.

J A Doudna1

  • 1Howard Hughes Medical Institute Department of Molecular Biophysics and Biochemistry Yale University New Haven, Connecticut, 06511, USA. doudna@csb.yale.edu.

Current Biology : CB
|October 26, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Methods to crystallize RNA.

Current protocols in nucleic acid chemistry·2008
Same author

Structure of Dicer and mechanistic implications for RNAi.

Cold Spring Harbor symposia on quantitative biology·2007
Same author

Mechanisms of internal ribosome entry in translation initiation.

Cold Spring Harbor symposia on quantitative biology·2003
Same author

Creative catalysis: pieces of the RNA world jigsaw.

Trends in biochemical sciences·2001
Same author

Direct pK(a) measurement of the active-site cytosine in a genomic hepatitis delta virus ribozyme.

Journal of the American Chemical Society·2001
Same author

Ribozyme structures and mechanisms.

Annual review of biophysics and biomolecular structure·2001
Same journal

Hunting ecology predicts eye arrangements in the modular visual system of spiders.

Current biology : CB·2026
Same journal

Sub-second fluctuations between top-down and bottom-up modes distinguish diverse human brain states.

Current biology : CB·2026
Same journal

Queen bees offload pesticide burden to eggs when social buffering is overwhelmed.

Current biology : CB·2026
Same journal

Pitch selectivity in ferret auditory cortex.

Current biology : CB·2026
Same journal

A cell size-dependent competition between geometry and polarity governs nuclear and spindle positioning in early embryos.

Current biology : CB·2026
Same journal

Trophic cascades drive sustainability in the agricultural heritage rice-fish coculture system.

Current biology : CB·2026
See all related articles

High-resolution X-ray crystallography revealed ribosome subunit structures, detailing RNA-protein interactions and substrate orientations at the interface of the intact ribosome.

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • The ribosome is a complex molecular machine responsible for protein synthesis.
  • Understanding ribosome structure is crucial for deciphering its function in translation.
  • Previous structural studies provided lower-resolution insights into ribosome architecture.

Purpose of the Study:

  • To determine the high-resolution structures of the ribosome and its subunits.
  • To elucidate the detailed interactions between ribosomal RNA and proteins within the subunits.
  • To visualize the precise orientations of substrates at the subunit interface in the intact ribosome.

Main Methods:

  • X-ray crystallography was employed to obtain high-resolution structural data.
  • Crystallographic data were processed and analyzed to build atomic models of the ribosome and its subunits.

Related Experiment Videos

Main Results:

  • The study achieved resolutions sufficient to visualize atomic details of the ribosome.
  • Detailed interactions between RNA and protein components within each subunit were revealed.
  • The orientations of various substrates at the interface between the two subunits in the intact ribosome were precisely determined.

Conclusions:

  • The determined structures provide unprecedented atomic-level insights into ribosome architecture and function.
  • These findings advance our understanding of the molecular mechanisms underlying protein synthesis.
  • The high-resolution data serve as a valuable resource for future ribosome-related research.