Jove
Visualize
Contact Us

Related Experiment Videos

Histone H1(o) expression in the developing cat retina.

J J Miguel-Hidalgo1, C Puckett Robinson

  • 1Department of Psychology, University of California, Davis, CA, USA. puckettr@hsc.usc.edu

Brain Research. Developmental Brain Research
|October 28, 1999
PubMed
Summary

Histone H1(o) expression in cat retina occurs before cell differentiation and light exposure, differing from mouse studies. This suggests light and differentiation do not trigger H1(o) in cats.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prenatal infection decreases calbindin, decreases Purkinje cell volume and density and produces long-term motor deficits in Sprague-Dawley rats.

Developmental neuroscience·2010
Same author

Gliogenesis and glial pathology in depression.

CNS & neurological disorders drug targets·2007
Same author

Neuroprotection by memantine against neurodegeneration induced by beta-amyloid(1-40).

Brain research·2002
Same author

SB-271046 (SmithKline Beecham).

Current opinion in investigational drugs (London, England : 2000)·2001
Same author

Glial fibrillary acidic protein immunoreactivity in the prefrontal cortex distinguishes younger from older adults in major depressive disorder.

Biological psychiatry·2000
Same author

Immunohistochemistry of neural markers for the study of the laminar architecture in celloidin sections from the human cerebral cortex.

Journal of neuroscience methods·1999
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Neuroscience

Background:

  • Histone H1(o) is a linker histone involved in gene regulation.
  • Previous studies linked H1(o) expression to cell differentiation in mouse retina and rat brain.
  • Light exposure was suggested to trigger H1(o) expression in the mouse retina.

Purpose of the Study:

  • To investigate the relationship between H1(o) protein appearance and retinal cell/layer formation in cats.
  • To determine if H1(o) expression in the cat retina is dependent on light or postnatal development.

Main Methods:

  • Immunoreactivity analysis of H1(o) protein in developing cat retina.
  • Correlation of H1(o) expression timing with cellular differentiation and light exposure.

Related Experiment Videos

Main Results:

  • H1(o) immunoreactivity was observed in ganglion, amacrine, and prospective bipolar cells before birth and terminal differentiation.
  • H1(o) expression in the cat retina precedes light exposure.
  • H1(o) expression is not required for terminal differentiation of retinal cells in cats.
  • H1(o) expression in cats is not dependent on light exposure.

Conclusions:

  • Histone H1(o) expression in the cat retina is independent of terminal cell differentiation and light exposure.
  • Findings contradict previous studies in mice, indicating species-specific regulation of H1(o).
  • H1(o) is not universally required for light-induced differentiation in retinal cells across species.