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Identification of synthetic by-products in combinatorial libraries using high performance liquid
J L Aubagnac1, M Amblard, C Enjalbal
1UMR 5810 - Laboratoire des Aminoacides, Peptides et Protéines - Universités Montpellier I et II, Place E. Bataillon, 34095 Montpellier Cédex 05, France. aubagnac@univ-montp2.fr
Abstract:
High performance liquid chromatography (HPLC), electrospray ionization mass spectrometry (ESI) and high performance liquid chromatography coupled to mass spectrometry (LC-MS) were used to analyze randomly chosen samples from parallel syntheses carried out on derivatized polypropylene crowns compatible with a Multipin solid support system. Side-reactions and by-products were clearly identified, and the yields of the expected molecules were unexpectedly low for most samples. LC-MS was superior to HPLC with absorbance detection or electrospray mass spectrometry alone for determining the identity and purity of each desired combinatorial compounds.