Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rod multifocal electroretinograms in mice.

S Nusinowitz1, W H Ridder, J R Heckenlively

  • 1Jules Stein Eye Institute, Los Angeles, California 90024, USA. nusinowitz@jsei.ucla.edu

Investigative Ophthalmology & Visual Science
|November 5, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retinal Structure in Pre-Clinical Age-Related Macular Degeneration.

Current eye research·2017
Same author

exomeSuite: Whole exome sequence variant filtering tool for rapid identification of putative disease causing SNVs/indels.

Genomics·2014
Same author

Establishing baseline rod electroretinogram values in achromatopsia and cone dystrophy.

Documenta ophthalmologica. Advances in ophthalmology·2012
Same author

New concept: treating nonproliferative diabetic retinopathy with light adaptation of rods during sleep.

Eye (London, England)·2011
Same author

Analysis of autofluorescent retinal images and measurement of atrophic lesion growth in Stargardt disease.

Experimental eye research·2010
Same author

Interoperator variability in images obtained by laser polarimetry of the nerve fiber layer.

Journal of glaucoma·2009

Researchers successfully recorded rod multifocal electroretinograms (ERGs) in mice, creating detailed functional maps of the retina. This technique aids in studying retinal diseases and testing therapies in mouse models.

Area of Science:

  • Ophthalmology
  • Neuroscience
  • Vision Science

Background:

  • Rod-mediated vision is crucial for low-light conditions.
  • Multifocal electroretinography (mfERG) assesses retinal function.
  • Developing mouse models for retinal diseases requires precise functional assessment tools.

Purpose of the Study:

  • To establish the feasibility of recording rod-specific multifocal electroretinograms (mfERGs) in the mouse eye.
  • To optimize stimulus conditions for reliable rod mfERG recording in mice.
  • To validate the technique for mapping retinal function and detecting localized defects.

Main Methods:

  • Multifocal ERGs were recorded from C57BL/6J mice using a hexagonal array and blue stimuli.
  • Stimulus intensity, size, surround intensity, and adaptation levels were systematically varied.

Related Experiment Videos

  • Cone intrusion was assessed by altering adaptation levels; response isolation was confirmed via full-field ERGs and laser-induced defects.

    • Main Results:

    • Rod mfERGs were successfully recorded, showing clear waveforms under optimized conditions.
    • Response amplitudes were sensitive to flash intensity, stimulus size, surround intensity, and adaptation level.
    • The technique accurately mapped laser-induced retinal defects, demonstrating its spatial resolution and utility.

    Conclusions:

    • Rod mfERG recording is feasible in mice, providing topographical retinal function maps.
    • This technique offers practical spatial resolution for studying mouse models of human retinal diseases.
    • It is valuable for tracking disease progression and evaluating therapeutic interventions in preclinical studies.