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Related Experiment Videos

Factor XI messenger RNA in human platelets.

D Martincic1, V Kravtsov, D Gailani

  • 1Departments of Pediatrics, Pathology, and Medicine, Vanderbilt University, Nashville, TN 37232-6305, USA.

Blood
|November 24, 1999
PubMed
Summary
This summary is machine-generated.

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Platelets contain wild-type Factor XI (FXI) mRNA, identical to liver mRNA. This suggests FXI protein may be present in platelets and released upon activation, explaining variable bleeding in FXI deficiency.

Area of Science:

  • Hematology
  • Molecular Biology
  • Genetics

Background:

  • Congenital Factor XI (FXI) deficiency causes variable bleeding, poorly correlating with coagulation tests.
  • Platelets may possess FXI activity, potentially explaining this variability.
  • A distinct 220 kD platelet protein cross-reacts with FXI antibodies, hypothesized to be from alternatively spliced mRNA.

Purpose of the Study:

  • To investigate the source of FXI activity in platelets.
  • To determine if platelets contain FXI mRNA and if it differs from plasma FXI mRNA.
  • To test the hypothesis of an alternatively spliced FXI mRNA in platelets.

Main Methods:

  • Reverse transcription polymerase chain reaction (RT-PCR) to analyze RNA from platelets, leukocytes, and bone marrow.

Related Experiment Videos

  • Sequencing of PCR products to confirm FXI mRNA identity.
  • Analysis of leukemia cell lines with megakaryocyte features.

    • Main Results:

    • Wild-type FXI mRNA, identical to liver mRNA, was detected in platelets and bone marrow, but not leukocytes.
    • Sequencing confirmed the platelet FXI mRNA is the same as the liver (wild-type) version.
    • The data do not support the alternative splicing hypothesis for the platelet protein.

    Conclusions:

    • Platelets contain wild-type FXI mRNA, suggesting the presence and potential release of FXI protein.
    • This finding may explain the variable bleeding patterns observed in patients with Factor XI deficiency.
    • The 220 kD platelet protein is unlikely to be derived from an alternatively spliced FXI mRNA.