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Definitive evidence that the murine C-type virus inducing locus Akv-1 is viral genetic material
Abstract:
DNA of the AKR mouse contains a set of murine leukemia virus sequences that are not present in DNA of the NIH Swiss mouse. NIH mice partially congenic for the AKR murine-leukemia-virus-inducing locus Akv-1 contain this set of sequences, and, in a three-point cross segregating for Akv-1 on an NIH background, the sequences segregated with Akv-1. It is concluded that the Akv-1 locus contains viral sequences.
Insights
AKR mouse DNA harbors unique murine leukemia virus sequences absent in NIH Swiss mice. These viral sequences are located within the Akv-1 locus, as demonstrated by genetic segregation analysis.
Area of Science:
- Genetics
- Virology
- Mouse Models
Background:
- Murine leukemia viruses (MLVs) are retroviruses known to infect mice.
- Genetic differences exist between mouse strains, influencing disease susceptibility.
- The AKR mouse strain is known to carry endogenous MLV sequences.
Purpose of the Study:
- To investigate the genetic basis of MLV sequence presence in AKR mice.
- To determine if specific genetic loci are associated with MLV sequences.
- To characterize the inheritance pattern of these viral sequences.
Main Methods:
- Comparative DNA analysis between AKR and NIH Swiss mouse strains.
- Congenic mouse strain development for genetic mapping.
- Three-point genetic cross analysis to track gene and sequence segregation.
Main Results:
- AKR mouse DNA contains specific MLV sequences not found in NIH Swiss mice.
- NIH Swiss mice engineered to be partially congenic for the Akv-1 locus possess these MLV sequences.
- In genetic crosses, the MLV sequences segregated concordantly with the Akv-1 locus.
Conclusions:
- The Akv-1 locus in mice contains endogenous viral sequences.
- This finding provides a genetic marker for MLV presence in mouse populations.
- Understanding the genetic location of viral sequences is crucial for studying host-virus interactions.