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Updated: Jul 28, 2026
07:07
Murine Model of Intestinal Ischemia-reperfusion Injury
Published on: May 11, 2016
Effects of intestinal resection on enterocyte apoptosis
1Surgical Service, Omaha VA Medical Center, University of Nebraska Medical Center, Omaha, NE 68198-3280, USA.
Summary
Intestinal resection increases cell proliferation and apoptosis. While villus tip apoptosis remains high, crypt apoptosis normalizes, suggesting independent regulation during intestinal adaptation.
Area of Science:
- Gastroenterology
- Cell Biology
- Regenerative Medicine
Background:
- Intestinal resection triggers adaptive enterocyte hyperplasia.
- Early adaptive responses involve increased crypt cell proliferation and apoptosis.
Purpose of the Study:
- To investigate changes in proliferation and apoptosis later in the intestinal adaptation period post-resection.
- To understand the temporal regulation of apoptosis in crypt and villus compartments.
Main Methods:
- Studied rabbits 7, 14, and 21 days after 50% proximal intestinal resection.
- Assessed morphometry, crypt cell production rate (CCPR), and DNA fragmentation (apoptosis).
- Analyzed apoptotic cells via morphologic evaluation in crypt and villus compartments.
Main Results:
- Villus height and crypt depth significantly increased by day 21.
- CCPR remained elevated throughout the study period.
- Villus tip apoptosis was significantly increased at 21 days, while crypt apoptosis returned to baseline by this time.
Conclusions:
- Villus tip cell apoptosis remains elevated late in post-resection adaptation.
- Crypt cell apoptosis normalizes while proliferation is still stimulated, indicating independent regulation.
- Apoptosis in crypt and villus compartments appears to be independently regulated during intestinal adaptation.
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