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Related Experiment Videos

Intraperitoneal immunity and pneumoperitoneum.

E G Chekan1, C Nataraj, E M Clary

  • 1Department of Surgery, Duke University Medical Center, Post Office Box 3110, Durham, NC 27710, USA.

Surgical Endoscopy
|November 11, 1999
PubMed
Summary
This summary is machine-generated.

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Carbon dioxide (CO(2)) pneumoperitoneum impairs immune cell function in mice more than helium (He) insufflation. This immunosuppression resolves by day 5, indicating a temporary effect on cell-mediated immunity.

Area of Science:

  • Immunology
  • Surgical Research
  • Microbiology

Background:

  • Carbon dioxide (CO(2)) pneumoperitoneum may depress intraperitoneal immunity.
  • In vitro studies show CO(2) decreases peritoneal macrophage function.
  • An effective in vivo assessment of CO(2) pneumoperitoneum's effect on immunity is lacking.

Purpose of the Study:

  • To evaluate the in vivo immune competence of mice following CO(2) vs. helium (He) pneumoperitoneum.
  • To assess the ability of mice to clear Listeria monocytogenes (LM) after different insufflation methods.
  • To compare the impact of CO(2) pneumoperitoneum, He pneumoperitoneum, and laparotomy on cell-mediated immunity.

Main Methods:

  • Eighty-five C57Bl/6 mice were divided into four groups: CO(2) insufflation, He insufflation, laparotomy (Lap), and control.

Related Experiment Videos

  • Mice received an intraperitoneal inoculation of Listeria monocytogenes (LM) immediately postoperatively.
  • Bacterial burden in spleens and livers was quantified on postoperative days 3 and 5.

    • Main Results:

    • On day 3, the CO(2) group showed significantly higher bacterial burden compared to He and control groups.
    • Laparotomy also resulted in a significantly higher bacterial burden compared to controls on day 3.
    • No significant differences in bacterial burden were observed between any groups on day 5.

    Conclusions:

    • CO(2) pneumoperitoneum significantly impairs cell-mediated intraperitoneal immunity more than He pneumoperitoneum and controls on day 3.
    • Laparotomy also impairs intraperitoneal immunity on day 3 compared to controls.
    • Intraperitoneal immunosuppression induced by these procedures resolves by day 5.