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Urinary thymine dimers and 8-oxo-2'-deoxyguanosine in psoriasis
J Ahmad1, M S Cooke, A Hussieni
1Division of Chemical Pathology, Centre for Mechanisms of Human Toxicity, PO Box 138, University of Leicester, Lancaster Road, Leicester, UK.
FEBS Letters
|November 11, 1999
Summary
Urinary thymine dimers, a marker of DNA damage, are significantly elevated in psoriasis patients undergoing PUVA therapy. This simple, non-invasive assay can help monitor treatment risks and optimize psoriasis therapy.
Area of Science:
- Dermatology
- Molecular Biology
- Biochemistry
Background:
- Psoralen plus ultraviolet A (PUVA) is a highly effective psoriasis treatment.
- PUVA therapy increases skin cancer risk due to DNA damage.
- There is a need for non-invasive methods to assess treatment-induced DNA damage.
Purpose of the Study:
- To assess urinary thymine dimers (T<>T) and 8-oxo-2'-deoxyguanosine (8-OHdG) as biomarkers of DNA damage in psoriasis patients.
- To evaluate the utility of these biomarkers for monitoring PUVA therapy risks.
Main Methods:
- Collected and analyzed urine samples from psoriasis patients and control groups.
- Measured urinary levels of thymine dimers and 8-OHdG.
- Corrected biomarker levels for urine concentration.
Main Results:
- Psoriatic patients showed significantly higher urinary thymine dimer levels compared to controls (P<0.0001).
- No significant difference in urinary 8-OHdG levels was observed between psoriatic, atopic dermatitis, and control groups.
Conclusions:
- Urinary thymine dimers serve as a sensitive, non-invasive biomarker for DNA damage induced by PUVA therapy in psoriasis.
- This assay can facilitate early risk assessment and optimization of PUVA treatment for reduced adverse effects.