Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

The ion channel switch biosensor.

G Woodhouse¹, L King, L Wieczorek

¹Cooperative Research Centre for Molecular Engineering and Technology, Australian Membrane and Biotechnology Research Institute, 126 Greville Street, Chatswood, NSW 2067, Australia.

Journal of Molecular Recognition : JMR

|November 11, 1999

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Half-life and gamma-ray emission intensity measurements for <sup>227</sup>Th.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2026

Same author

Moving forwards with team care.

British dental journal·2024

Same author

Family poverty over the early life course and adult experiences of intimate partner violence: a cohort study.

Public health·2024

Same author

OpenHands: An Open-Source Statistical Shape Model of the Finger Bones.

Annals of biomedical engineering·2024

Same author

Assessing domain match and feasibility of candidate instruments matching with OMERACT endorsed domains to measure flare in knee and hip osteoarthritis.

Seminars in arthritis and rheumatism·2024

Same author

Absolute emission intensities of the gamma rays from the decay of <sup>224</sup>Ra and <sup>212</sup>Pb progenies and the half-life of the <sup>212</sup> Pb decay.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2024

Same journal

3D-Printed Structures Versus Drilled Cavities: A Comparison of Microconfinement Methods for Rheological Characterisation of Multicellular Aggregates.

Journal of molecular recognition : JMR·2026

Same journal

Temperature Dependent Motions of N-Terminal Loop in PD-1 Determine the Affinity Towards Nivolumab.

Journal of molecular recognition : JMR·2026

Same journal

AFM Force Volume for Extracellular Vesicle Detection and Membrane Blebbing Analysis Through Mechanical Signature Mapping.

Journal of molecular recognition : JMR·2026

Same journal

Induced Circular Dichroism From the Binding of Achiral Bivalent Ligands to Transthyretin.

Journal of molecular recognition : JMR·2026

Same journal

Design of Multi-Epitope DIVA-Compatible Vaccine Candidate Against Canine Parvovirus 2.

Journal of molecular recognition : JMR·2026

Same journal

Artificial Intelligence Clustering Approach for Force Mapping Analysis of Polyacrylic Acid (PAA)/Polyethylene Oxide (PEO) Polymer Brushes for Biosensor Applications.

Journal of molecular recognition : JMR·2026

See all related articles

This study introduces a novel biosensor for direct molecular interaction measurement using ion-channel electrical signals. It achieves high sensitivity for detecting various analytes in complex biological samples.

Area of Science:

Biomolecular Engineering
Biosensor Technology
Molecular Interactions

Background:

Direct measurement of functional molecular interactions is crucial for understanding biological processes.
Existing biosensor technologies often face limitations in sensitivity and applicability to complex biological matrices.
Tethered bilayer membranes offer a biomimetic platform for studying surface-bound molecular events.

Purpose of the Study:

To develop and characterize a novel biosensor technology for direct measurement of functional molecular interactions.
To leverage electrical transduction of chemically modified ion-channels for high-sensitivity analyte detection.
To demonstrate the sensor's capability in measuring molecular recognition within complex biological samples.

Main Methods:

Related Experiment Videos

Utilized a tethered bilayer membrane as the sensor surface.
Employed chemically modified ion-channels for electrical transduction of molecular binding events.
Developed a quantitative model incorporating 3D and 2D molecular interaction dynamics.

Main Results:

Achieved high sensitivity in analyte detection due to significant ion flux through the ion channels.
Demonstrated successful molecular recognition measurements in complex biological matrices like blood and sera.
Quantified activity and concentration of diverse analytes including bacteria, DNA, proteins, and drugs.

Conclusions:

The described biosensor technology enables direct, sensitive measurement of functional molecular interactions.
The biomimetic sensor surface facilitates molecular recognition in complex biological environments without sensitivity loss.
The developed quantitative model accurately describes biosensor performance based on molecular interaction geometries.