Large-scale applications of NAD(P)-dependent oxidoreductases: recent developments

Area of Science:

• Biocatalysis and Enzyme Engineering
• Organic Synthesis
• Green Chemistry

Background:

• NAD(P)-dependent dehydrogenases are valuable biocatalysts for producing chiral compounds.
• These enzymes exhibit high enantioselectivity in reducing ketones and keto acids.
• Economic viability necessitates efficient regeneration of the cofactor NAD(P)H during reactions.

Purpose of the Study:

• To outline the essential components for preparative applications of NAD(P)-dependent dehydrogenases.
• To highlight the integration of enzymatic catalysis with coenzyme regeneration and reaction engineering.

Main Methods:

• Utilizing NAD(P)-dependent dehydrogenases for enantioselective reduction of prochiral substrates.
• Implementing simultaneous regeneration of the nicotinamide cofactor (NAD(P)H).
• Applying suitable reaction-engineering techniques for scale-up and efficiency.

Main Results:

• Demonstrated the feasibility of combining enzymes, coenzyme regeneration, and reaction engineering.
• Achieved high enantioselectivity in the synthesis of chiral alcohols, hydroxy acids, and amino acids.

Conclusions:

• The combination of an appropriate enzyme, efficient coenzyme regeneration, and optimized reaction engineering is crucial for the preparative synthesis of chiral compounds.
• This integrated approach enhances the economic feasibility and applicability of biocatalytic reductions using NAD(P)-dependent dehydrogenases.