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Autoimmune and non-autoimmune thyroid diseases have different patterns of cellular HLA class II expression
D E Zantut-Wittmann1, L H Boechat, G A Pinto
1Department of Pathologic Anatomy, School of Medical Sciences-State University of Campinas, Brazil. hwittmann@TRT15.gov.br
Context:
Surface HLA-DR antigen is usually only expressed by antigen-presenting cells (APC). In autoimmune thyroid disease, follicle cells function as APC, thus expressing HLA-DR. However, non-autoimmune thyroid diseases may also express surface class II antigens.
Objective:
To evaluate the presence and pattern of HLA class II expression in autoimmune and non-autoimmune thyroid disorders.
Design:
Retrospective: histopathological and immunohistochemical analysis.
Location:
Referral center, university hospital.
Sample:
Ten histologically normal thyroids, 11 Graves' disease, 7 Hashimoto's thyroiditis, 10 atoxic multinodular goiter and 3 toxic adenomas were analyzed by immunohistochemistry, using a monoclonal antibody anti-HLA-DR.
Main Measurements:
The presence of these antigens in thyroid follicular cells and their relation to inflammatory infiltrate was evaluated. The pattern of HLA-DR expression in thyroid follicular cells was analyzed: membrane, cytoplasmic or both.
Results:
Although HLA-DR antigens were sparsely present in one of the 8 normal thyroids, in 6 of the 9 atoxic multinodular goiter and in 2 of the 3 toxic adenomas a net positivity could be seen in large areas. In all 5 Hashimoto's thyroiditis and in 7 of the 10 Graves' disease cases. This expression occurred in follicle cells either in contact with inflammatory cells or not. In non-autoimmune thyroid disease, HLA-DR positivity was essentially cytoplasmic, whereas in Graves' disease and Hashimoto thyroiditis it was mainly in cell membranes.
Conclusions:
It is suggested that the HLA class II expression on the surface of follicle cells could be related to auto-antigen presentation to the immune system by these cells, leading to inflammation.