Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Number e as a Limit01:29

The Number e as a Limit

102
The number e is a fundamental constant in calculus, playing a central role in describing continuous change, particularly exponential growth. It is most naturally defined through its relationship with the natural logarithm, which is the inverse of the exponential function with base e. This relationship allows e to be characterized using basic principles of differentiation rather than as an arbitrary numerical constant.A key property of the natural logarithm function, ln x, is that its derivative...
102
Types of Limits I01:23

Types of Limits I

209
Limits are a key mathematical concept for understanding how functions behave as their input approaches specific values, particularly when the function is undefined. They help reveal trends and discontinuities by examining the values a function approaches rather than its actual value.One-sided limits focus on the direction from which a value is approached. When a function behaves differently depending on whether the input approaches from the left or the right, the two one-sided limits may not...
209
Limit Laws I01:25

Limit Laws I

233
Limit laws provide essential tools for analyzing how functions behave as their input approaches a specific value. These laws are particularly useful when dealing with combinations of functions, provided the individual limits exist. The Sum and Difference Laws state that the limit of the sum or difference of two functions equals the sum or difference of their respective limits:The Product Law asserts that the limit of the product of two functions equals the product of their individual limits:A...
233
Apoptosis01:30

Apoptosis

15.3K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
15.3K
Limiting Reactant02:27

Limiting Reactant

70.6K
The relative amounts of reactants and products represented in a balanced chemical equation are often referred to as stoichiometric amounts. However, in reality, the reactants are not always present in the stoichiometric amounts indicated by the balanced equation.
70.6K
Cross-Sectional Research01:50

Cross-Sectional Research

12.7K
In cross-sectional research, a researcher compares multiple segments of the population at the same time. If they were interested in people's dietary habits, the researcher might directly compare different groups of people by age. Instead of following a group of people for 20 years to see how their dietary habits changed from decade to decade, the researcher would study a group of 20-year-old individuals and compare them to a group of 30-year-old individuals and a group of 40-year-old...
12.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Octreotide and pasireotide effects on medullary thyroid carcinoma (MTC) cells growth, migration and invasion.

Molecular and cellular endocrinology·2020
Same author

The cytoskeleton actin binding protein filamin A impairs both IGF2 mitogenic effects and the efficacy of IGF1R inhibitors in adrenocortical cancer cells.

Cancer letters·2020
Same author

Approaching the transit time limit for high-precision spectroscopy on metastable CO around 6 μm.

Physical chemistry chemical physics : PCCP·2019
Same author

Cofilin is a mediator of RET-promoted medullary thyroid carcinoma cell migration, invasion and proliferation.

Molecular and cellular endocrinology·2019
Same author

Cytoskeleton actin-binding proteins in clinical behavior of pituitary tumors.

Endocrine-related cancer·2018
Same author

cAMP/PKA-induced filamin A (FLNA) phosphorylation inhibits SST2 signal transduction in GH-secreting pituitary tumor cells.

Cancer letters·2018

Related Experiment Video

Updated: Feb 13, 2026

The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning
05:00

The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning

Published on: September 5, 2018

19.8K

Staining apoptosis in paraffin sections. Advantages and limits.

F Mangili1, C Cigala, G Santambrogio

  • 1Anatomic Pathology Service, Istituto Scientifico H San Raffaele, Milan, Italy.

Analytical and Quantitative Cytology and Histology
|November 24, 1999
PubMed
Summary

The TdT-mediated dUTP nick end labeling (TUNEL) assay simplifies apoptotic cell identification in tissue sections. However, its results require careful interpretation alongside routine microscopy due to potential detection of non-apoptotic DNA fragmentation.

More Related Videos

Pancreatic Islet Embedding for Paraffin Sections
09:09

Pancreatic Islet Embedding for Paraffin Sections

Published on: June 29, 2018

13.6K
Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles
07:28

Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles

Published on: December 27, 2010

37.2K

Related Experiment Videos

Last Updated: Feb 13, 2026

The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning
05:00

The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning

Published on: September 5, 2018

19.8K
Pancreatic Islet Embedding for Paraffin Sections
09:09

Pancreatic Islet Embedding for Paraffin Sections

Published on: June 29, 2018

13.6K
Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles
07:28

Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles

Published on: December 27, 2010

37.2K

Area of Science:

  • Histopathology
  • Cell Biology
  • Biomolecular Assays

Background:

  • Apoptosis detection is crucial for understanding tissue pathology.
  • Routine methods for identifying apoptotic cells in fixed tissues have limitations.

Purpose of the Study:

  • To evaluate the advantages and limitations of the TUNEL assay for detecting apoptosis in paraffin-embedded tissue sections.

Main Methods:

  • Analysis of 265 human tissue samples (malignant and benign) using TUNEL.
  • Biparametric analysis combining TUNEL with proliferation markers (MIB-1) and cell lineage markers (cytokeratin, leukocyte common antigen).

Main Results:

  • TUNEL simplifies the identification of apoptotic nuclei while preserving tissue topography.
  • The method can detect cells with DNA fragmentation not specific to apoptosis and is sensitive to proteolytic digestion.
  • TUNEL can identify nuclei in necrotic areas, but this is distinguishable by morphology.
  • Biparametric staining aids in identifying the origin of apoptotic cells and allows simultaneous assessment of apoptosis and proliferation.

Conclusions:

  • TUNEL is a valuable method for detecting apoptotic nuclei in fixed, embedded tissue sections.
  • Results from TUNEL should be interpreted cautiously, considering its limitations, and ideally complemented by routine light microscopy assessment.