Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ventral coxofemoral luxation in poodle breeds: multicentre retrospective case series.

The Journal of small animal practice·2026
Same author

Global genomic diversity of Pseudomonas aeruginosa in bronchiectasis.

The Journal of infection·2024
Same author

The impact of side effect framing on COVID-19 booster vaccine intentions in an Australian sample.

Vaccine·2023
Same author

A near-deterministic mutational hotspot in Pseudomonas fluorescens is constructed by multiple interacting genomic features.

Molecular biology and evolution·2022
Same author

Pre-Exposure, But Not Overshadowing, Inhibits Nocebo Hyperalgesia.

The journal of pain·2021
Same author

Deceptive but not open label placebos attenuate motion-induced nausea.

Journal of psychosomatic research·2019
Same journal

Update on the progress in acrylamide and furan research. Proceedings of the DG Sanco/CIAA sponsored workshop "Acrylamide" and joint DG Sanco/EFSA/DG JRC workshop "Furan in food." March 16-17, 20006 and May 19, 2006, respectively. Brussels, Belgium.

Food additives and contaminants·2008
Same journal

Food additives and contaminants.

Food additives and contaminants·2007
Same journal

Index of authors---volume 24.

Food additives and contaminants·2007
Same journal

High-performance liquid chromatographic method for the simultaneous detection of the adulteration of cereal flours with melamine and related triazine by-products ammeline, ammelide, and cyanuric acid.

Food additives and contaminants·2007
Same journal

Mycotoxin occurrence and Aspergillus flavus soil propagules in a corn and cotton glyphosate-resistant cropping systems.

Food additives and contaminants·2007
Same journal

Occurrence and fate of Fusarium mycotoxins during commercial processing of oats in the UK.

Food additives and contaminants·2007
See all related articles

Related Experiment Video

Updated: Jun 24, 2026

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
10:01

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Published on: April 23, 2012

Ochratoxin A in dried vine fruit: method development and survey.

S MacDonald1, P Wilson, K Barnes

  • 1CSL Food Science Laboratory, Norwich Research Park, UK.

Food Additives and Contaminants
|November 24, 1999
PubMed
Summary
This summary is machine-generated.

Mycotoxin ochratoxin A is prevalent in UK dried vine fruits, with 88% of samples exceeding the detection limit. This study details a method for its accurate determination, finding no aflatoxins present.

More Related Videos

RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
09:44

RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem

Published on: December 21, 2015

Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds
10:24

Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds

Published on: April 19, 2024

Related Experiment Videos

Last Updated: Jun 24, 2026

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
10:01

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Published on: April 23, 2012

RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
09:44

RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem

Published on: December 21, 2015

Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds
10:24

Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds

Published on: April 19, 2024

Area of Science:

  • Food safety analysis
  • Mycotoxicology
  • Analytical chemistry

Background:

  • Mycotoxins, such as ochratoxin A (OA), pose a significant risk to human health.
  • Dried vine fruits are susceptible to fungal contamination and subsequent mycotoxin production.
  • Regulatory limits exist for mycotoxins in foodstuffs, necessitating reliable detection methods.

Purpose of the Study:

  • To develop and validate a method for determining ochratoxin A concentrations in dried vine fruits.
  • To assess the incidence and levels of ochratoxin A in retail dried vine fruits in the UK.
  • To screen for the presence of aflatoxins in the same samples.

Main Methods:

  • Acidic methanolic extraction of mycotoxins from dried vine fruits.
  • Immunoaffinity chromatography for selective clean-up of ochratoxin A.
  • High-performance liquid chromatography (HPLC) with UV detection for quantification.
  • HPLC coupled with mass spectrometry (MS) for identity confirmation.
  • Analysis of 60 retail samples of currants, raisins, and sultanas purchased in the UK.

Main Results:

  • The developed method achieved a limit of detection of 0.2 µg/kg for ochratoxin A with recoveries between 63-77% at 5 µg/kg.
  • Ochratoxin A was detected in 88% of the analyzed dried vine fruit samples (19/20 currants, 17/20 sultanas, 17/20 raisins).
  • The maximum ochratoxin A level found was 53.6 µg/kg; no aflatoxins were detected (limit of detection 0.2 µg/kg for B1, B2, G1, G2).

Conclusions:

  • Ochratoxin A is widespread in UK retail dried vine fruits, frequently exceeding the method's limit of detection.
  • The described method is effective for the accurate determination and confirmation of ochratoxin A in dried vine fruits.
  • Further monitoring and risk assessment are warranted due to the high incidence of ochratoxin A in these commodities.