Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CTP:phosphocholine cytidylyltransferase activation by oxidized phosphatidylcholines correlates with a decrease in

A E Drobnies1, B van Der Ende, J L Thewalt

  • 1Institute of Molecular Biology, Simon Fraser University, Burnaby, British Columbia, Canada.

Biochemistry
|November 26, 1999
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural Properties of Inverted Hexagonal Phase: A Hybrid Computational and Experimental Approach.

Langmuir : the ACS journal of surfaces and colloids·2020
Same author

Ionizable amino lipid interactions with POPC: implications for lipid nanoparticle function.

Nanoscale·2019
Same author

The course of the radial nerve in the distal humerus: A novel, anatomy based, radiographic assessment.

PloS one·2017
Same author

Phosphatidylcholine: cholesterol phase diagrams.

Biophysical journal·2009
Same author

Universal behavior of membranes with sterols.

Biophysical journal·2005
Same author

Regulation of CTP: phosphocholine cytidylyltransferase activity by the physical properties of lipid membranes: an important role for stored curvature strain energy.

Biochemistry·2001

Oxidized phosphatidylcholine activates the enzyme CTP:phosphocholine cytidylyltransferase (CT) by reducing membrane lipid order. This study identifies a specific oxidized lipid responsible for CT activation, revealing a novel mechanism for enzyme regulation.

Area of Science:

  • Biochemistry
  • Membrane Biology
  • Enzyme Regulation

Background:

  • CTP:phosphocholine cytidylyltransferase (CT) is a key enzyme in phosphatidylcholine synthesis, reversibly binding to membranes.
  • CT activity is regulated by membrane lipid composition, with oxidized lipids showing unique activation properties.
  • Previous studies indicated activation by oxidized phosphatidylcholine (PC) vesicles, but the mechanism was unclear.

Purpose of the Study:

  • To investigate the mechanism by which oxidized 1-palmitoyl-2-arachidonoylPC (PAPC) activates CT.
  • To determine the effect of oxidized PAPC on membrane lipid order.
  • To identify and characterize the specific oxidized lipid responsible for CT activation.

Main Methods:

  • Utilized multilamellar vesicles (MLVs) with varying concentrations of oxidized PAPC.

Related Experiment Videos

  • Employed (2)H NMR spectroscopy with perdeuterated lipids to measure lipid order parameters.
  • Purified and characterized the major oxidized lipid component using mass spectrometry and NMR.

    • Main Results:

    • Oxidized PAPC progressively activated CT, with full activation at 25% oxidized PC.
    • Increased oxidized PAPC content decreased lipid order parameters along the acyl chain.
    • The purified oxidized lipid, 1-palmitoyl-2-(11,15-dihydroxy)eicosatrienoylPC (dihydroxyPAPC), mimicked the effects of oxidized PAPC.
    • Egg sphingomyelin reversed both CT activation and lipid disordering.

    Conclusions:

    • CT activation by oxidized PAPC is mediated by lipid packing perturbations and reduced membrane lipid order.
    • This is the first study to correlate purified oxidized lipid effects on acyl chain orientational order with regulatory enzyme activation.
    • The findings reveal a novel mechanism for membrane-associated enzyme regulation through lipid oxidation.