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Related Experiment Videos

Sorting by diffusion: an asymmetric obstacle course for continuous molecular separation.

C F Chou1, O Bakajin, S W Turner

  • 1Department of Physics, Princeton University, Princeton, NJ 08544, USA.

Proceedings of the National Academy of Sciences of the United States of America
|November 26, 1999
PubMed
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This study introduces a microfabricated sieve for DNA separation, utilizing Brownian motion rectification. This continuous flow technique offers an alternative to traditional batch-mode gel electrophoresis for DNA analysis.

Area of Science:

  • Biophysics
  • Nanotechnology
  • Molecular Biology

Background:

  • Traditional DNA separation methods like gel electrophoresis operate in batch mode.
  • Analyzing DNA molecule motion requires precise control and high resolution.
  • Brownian motion presents challenges for controlled molecular manipulation.

Purpose of the Study:

  • To demonstrate a novel microfabricated sieve for separating DNA molecules based on size.
  • To investigate the rectification of Brownian motion for size-dependent molecular trajectories.
  • To establish a continuous-flow separation technique for DNA analysis.

Main Methods:

  • Fabrication of a microfluidic device with a two-dimensional lattice of asymmetric obstacles.
  • Observing the motion of different-sized DNA molecules (15-30 kbp) through the sieve.

Related Experiment Videos

  • Utilizing Brownian motion driven by an external force to direct molecular paths.

    • Main Results:

    • Demonstrated successful separation of DNA molecules by size using the microfabricated sieve.
    • Achieved a nominal 6% resolution by length for DNA molecules in the 15-30 kbp range.
    • Validated that molecular path depends on diffusion coefficient, influenced by obstacle arrangement.

    Conclusions:

    • The microfabricated sieve offers a continuous-flow method for DNA separation.
    • This technique provides an advancement over batch-mode gel electrophoresis.
    • The device shows potential for high-throughput, size-based DNA analysis.