Related Experiment Video
Updated: Oct 7, 2026

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
Published on: April 19, 2014
Tissue-inherent fate of GPI revealed by GPI-anchored GFP transgenesis
1Department of Social and Environmental Medicine, Osaka University Graduate School of Medicine, Suita, Japan. kondohg@mr-envi.med.osaka-u.ac.jp
Abstract:
To clarify the fate of glycosylphosphatidylinositol (GPI) in mammals, we developed GPI-anchored enhanced green fluorescent protein (EGFP-GPI) and transgenic mice carrying this fusion construct. When it was introduced to culture cells, the EGFP-GPI protein was correctly sorted to plasma membranes and microsomes depending on GPI biosynthesis. Transgenic mice carrying EGFP-GPI were found to show a broad transgene expression. Histologically, a prominent polarized localization of EGFP-GPI protein was observed in various epithelia, the nervous system and liver and secreted from some exocrine glands, as well as non-polarized presence in non-epithelial tissues, demonstrating a tissue-inherent manner of GPI sorting.
More Related Videos
10:41Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
Published on: February 17, 2017
10:36In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
Published on: March 22, 2017
Related Concept Videos
Reporter Genes
Commonly used reporter...
GPI Anchoring of Proteins in the ER Membrane
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...