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Related Experiment Videos

Visualizing multiple constrictions in spheroidal Escherichia coli cells.

A Zaritsky1, A Van Geel, I Fishov

  • 1Department of Life Sciences, Ben-Gurion University of the Negev, POB 653, Be'er-Sheva84105, Israel.

Biochimie
|November 26, 1999
PubMed
Summary
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Investigating Escherichia coli cell division, this study found that FtsZ ring assembly is crucial for division plane determination. Shortage of FtsZ in wide cells leads to tilted division planes, impacting cell morphology and division timing.

Area of Science:

  • Microbiology
  • Cell Biology
  • Bacterial Physiology

Background:

  • Escherichia coli cells elongate and divide via perpendicular planes.
  • Determining division planes in three dimensions requires simultaneous constrictions in large cells.
  • Cellular constraints, like the peptidoglycan sacculus, influence nucleoid segregation and division.

Purpose of the Study:

  • To investigate the mechanism of cell division plane determination in Escherichia coli.
  • To understand the role of FtsZ ring formation in division.
  • To explore how cell width and FtsZ availability affect division plane orientation.

Main Methods:

  • Inducing large, spheroidal cells using mecillinam treatment.
  • Manipulating chromosome replication rate via thymine-limitation and deoxyguanosine addition.

Related Experiment Videos

  • Visualizing FtsZ ring structures using monoclonal antibodies against FtsZ.

    • Main Results:

    • Wide cells exhibited arcs of FtsZ instead of complete rings, indicating a shortage.
    • Observed arcs were not parallel or perpendicular, suggesting tilted constriction planes.
    • Tilted planes align with a 3-D 'nucleoid segregation' model, relieving cylindrical constraints.

    Conclusions:

    • FtsZ availability is critical for proper division ring formation and plane determination.
    • A shortage of FtsZ in wide cells leads to altered division planes and prolonged division times.
    • Overexpressing FtsZ-GFP fusion protein may overcome FtsZ scarcity and normalize division.