Plasmid topologies that enhance the transformation efficiency of yeast

Hypervariability, suppressed recombination and the genetics of individuality.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2004

General method for plasmid construction using homologous recombination.

BioTechniques·1999

Development of the methylotrophic yeast Pichia methanolica for the expression of the 65 kilodalton isoform of human glutamate decarboxylase.

Yeast (Chichester, England)·1998

The VPS1 protein is a dynamin-like GTPase required for sorting proteins to the yeast vacuole.

Ciba Foundation symposium·1993

Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants.

Molecular biology of the cell·1992

The VPS1 protein, a homolog of dynamin required for vacuolar protein sorting in Saccharomyces cerevisiae, is a GTPase with two functionally separable domains.

The Journal of cell biology·1992

A refined qPCR duplexing method for the molecular sex determination of sturgeon (Acipenser genus), providing a validating, endogenous control.

BioTechniques·2026

A QC-guided framework for performance assessment in plate-based RNAi screening with fluorescence and metabolic readouts.

BioTechniques·2026

Detection of antisense oligonucleotides from biological samples by ligase detection reaction using T4 RNA ligase 2.

BioTechniques·2026

A PCR method to detect the mouse major histocompatibility complex H2-Ld gene that confers resistance to Toxoplasma encephalitis.

BioTechniques·2026

Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein.

BioTechniques·2026

Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup.

BioTechniques·2026

Zr6-Cluster-Embedded ZIF-8 Membranes Fabricated by Electrochemical Deposition for CO2/N2 and CO2/CH4 Separation.

Inorganic chemistry·2026

Modular 3D culture platform combining methylcellulose, testicular ECM, and Sertoli cells for in vitro spermatogenesis.

Iranian journal of basic medical sciences·2026

Beyond trial-and-error: Fast in silico optimization of electromembrane extraction methods based on machine learning and a desktop app.

Analytica chimica acta·2026

Hierarchical MXene@ZIF-67 heterostructure for selective adsorption of tetracycline antibiotics: Synergistic mechanism and application in SPME Arrow.

Analytica chimica acta·2026

SH3-like domains from Leuconostoc citreum ABK-1 alternansucrase enables efficient enzyme immobilization.

International journal of biological macromolecules·2026

A rapid two-step protocol for isolation of DNA-free, high-integrity RNA from gram-negative Bacteria.

Journal of microbiological methods·2026

Related Experiment Videos

Plasmid topologies that enhance the transformation efficiency of yeast

Related Concept Videos

Hypervariability, suppressed recombination and the genetics of individuality.

General method for plasmid construction using homologous recombination.

Development of the methylotrophic yeast Pichia methanolica for the expression of the 65 kilodalton isoform of human glutamate decarboxylase.

The VPS1 protein is a dynamin-like GTPase required for sorting proteins to the yeast vacuole.

Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants.

The VPS1 protein, a homolog of dynamin required for vacuolar protein sorting in Saccharomyces cerevisiae, is a GTPase with two functionally separable domains.

A refined qPCR duplexing method for the molecular sex determination of sturgeon (Acipenser genus), providing a validating, endogenous control.

A QC-guided framework for performance assessment in plate-based RNAi screening with fluorescence and metabolic readouts.

Detection of antisense oligonucleotides from biological samples by ligase detection reaction using T4 RNA ligase 2.

A PCR method to detect the mouse major histocompatibility complex H2-Ld gene that confers resistance to Toxoplasma encephalitis.

Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein.

Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup.

Zr6-Cluster-Embedded ZIF-8 Membranes Fabricated by Electrochemical Deposition for CO2/N2 and CO2/CH4 Separation.

Modular 3D culture platform combining methylcellulose, testicular ECM, and Sertoli cells for in vitro spermatogenesis.

Beyond trial-and-error: Fast in silico optimization of electromembrane extraction methods based on machine learning and a desktop app.

Hierarchical MXene@ZIF-67 heterostructure for selective adsorption of tetracycline antibiotics: Synergistic mechanism and application in SPME Arrow.

SH3-like domains from Leuconostoc citreum ABK-1 alternansucrase enables efficient enzyme immobilization.

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Plasmid topologies that enhance the transformation efficiency of yeast

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