Related Experiment Video
Updated: Oct 8, 2026

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
The structure of the core part of Proteus vulgaris OX2 lipopolysaccharide
1Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark.
Abstract:
The identity of a novel structural component, an open-chain acetalic linkage, in the core part of the lipopolysaccharide (LPS) from Proteus vulgaris serotype OX2 has been determined by extensive NMR spectroscopic analysis of fragments isolated after mild acid hydrolysis of the intact LPS. The open-chain N-acetylgalactosamine fragment is substituted in the 4-position by non-stoichiometric amounts of a beta-galactopyranose residue and the overall structure of the core is as follows: [formula: see text] All sugars except the N-acetylgalactosamine are in the pyranose form, alpha-Hep refers to L-glycero-alpha-D-manno-heptopyranose and alpha-DDHep to D-glycero-alpha-D-manno-heptopyranose. Bold italics indicate non-stoichiometric substituents.
More Related Videos
12:57Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
Published on: September 17, 2013
10:24Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii
Published on: April 10, 2020
Related Concept Videos
Structure of Lipids
Structure of Lipids
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Structure of Lipids
Peptidoglycan Synthesis
Formation of Lipopolysaccharides