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CD14 expression by human mononuclear phagocytes is modulated by Clostridium difficile toxin B
J C Siffert1, C D Müller, S Dumont
1LPCCNM-UPRES 2308, Illkirch, faculté de pharmacie, université Louis Pasteur, France.
Microbes and Infection
|December 3, 1999
Summary
Clostridium difficile Toxin B alters CD14 expression on immune cells. Monocytes and macrophages show increased CD14 levels, but the effect varies with cell type and treatment duration.
Area of Science:
- Microbiology
- Immunology
- Cell Biology
Background:
- Clostridium difficile Toxin B causes pseudomembranous colitis.
- Toxin B alters cell morphology and membrane surface area.
- Toxin B and lipopolysaccharide synergistically stimulate TNF-alpha production.
Purpose of the Study:
- Investigate the effect of Toxin B on CD14 expression.
- Analyze CD14 modulation in monocytes and macrophages.
Main Methods:
- Flow cytometry was used to analyze CD14 expression.
- Monocytes and macrophages were treated with Toxin B.
Main Results:
- Toxin B treatment increased CD14 expression in monocytes after 5 hours.
- After 24 hours, monocyte CD14 expression decreased, but remaining cells showed higher levels.
- Toxin B significantly increased CD14 expression in macrophages, an effect sustained for at least 24 hours.
Conclusions:
- Toxin B modulates CD14 expression in a differentiation-dependent manner.
- The immune response to Toxin B may vary based on cell activation status.