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Stability of methacholine chloride in isotonic sodium chloride using a capillary electrophoresis assay
S Henn1, P Monfort, J H Vigneron
1Centre Hospitalier Universitaire de Brabois Adultes, Service Pharmacie, Nancy, France.
Objective:
To investigate the stability of methacholine chloride in 0.9% sodium chloride solutions.
Method:
Methacholine powder was mixed with diluent to a final concentration of 5 and 10 mg/ml. Duplicates of each admixture were divided and stored in glass vials at 25 degrees C, 4 degrees C and -20 degrees C for 12 months. At appropriate times intervals, samples were removed from solutions and analysed. Methacholine concentrations were measured using a high performance capillary electrophoresis assay.
Results:
No colour or other visual changes were seen in any sample. However, an additional peak was observed in some samples.
Conclusion:
Methacholine chloride solutions 5 mg/ml were stable in isotonic sodium chloride after refrigeration or freezing over a period of one year; methacholine chloride solutions 10 mg/ml were stable for one year after freezing. The solutions stored at ambient temperature were stable for 35 days and for less than 14 days, respectively, for the 5 and the 10 mg/ml solutions.