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Updated: Aug 25, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
Sheathless preconcentration-capillary zone electrophoresis-mass spectrometry applied to peptide analysis
1National Institute of Public Health and the Environment, Bilthoven, The Netherlands.
Abstract:
A reliable capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry system has been developed for the sensitive analysis and sequencing of peptides. The system comprises (1) a zero dead volume on-line sample preconcentration interface allowing over 100-fold increase in sample volume introduction and (2) a zero-dead volume, durable electrospray emitter yielding stable, low background electrospray signals, both proving not to compromize the electrophoretic performance. Sub-fmol sensitivities were obtained in applications to complex peptide samples derived from tryptic digestion of proteins and naturally processed major histocompatibility complex class I associated peptides.
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