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Chromosome constitution of cells from human gingiva
Pharmacology and Therapeutics in Dentistry
|January 1, 1975
Summary
Normal gingival tissues cultured in vitro developed fibroblast-like cells with abnormal chromosome numbers (heteroploidy) after 2 months. This genotypic variation occurred despite starting with normal diploid cells.
Area of Science:
- Cell biology
- Genetics
- Tissue engineering
Background:
- Normal gingival tissues possess a diploid chromosomal complement.
- In vitro cell culture techniques allow for the study of cellular behavior over time.
Purpose of the Study:
- To investigate cellular and chromosomal changes in normal gingival tissues during serial in vitro culture.
- To identify the point at which genotypic variation occurs after explantation.
Main Methods:
- Serial transfer of normal gingival tissue cultures.
- Monitoring of mitosis and cellular morphology.
- Karyotypic analysis of cultured cells.
Main Results:
- Out of 16 specimens, 9 were successfully cultured, with 7 maintained for over 50 weeks.
- After 2 months in culture, cells exhibited fibroblast-like morphology.
- Cultured cells consistently displayed a heteroploid chromosomal complement, characterized by extrasomy of chromosomes from all groups.
- Initial cells before culturing had a normal diploid number of 46 chromosomes.
Conclusions:
- Serial in vitro culture of normal gingival tissues leads to significant chromosomal alterations.
- Fibroblast-like cells with heteroploidy emerge and persist in long-term cultures.
- The study demonstrates in vitro genotypic variation in gingival tissue-derived cells.