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Related Experiment Videos

An integrated process for biomolecule isolation and purification.

X P Dai1, R G Luo, K K Sirkar

  • 1Center for Membrane Technologies, Department of Chemical Engineering, New Jersey Institute of Technology, Newark, New Jersey 07102, USA.

Biotechnology Progress
|December 10, 1999
PubMed
Summary
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This study introduces a novel cyclic process device that integrates ultrafiltration and chromatography for biomolecule purification. This single-device approach reduces cost and product loss in biomolecule isolation.

Area of Science:

  • Biochemistry
  • Biotechnology
  • Chemical Engineering

Background:

  • Traditional biomolecule purification involves multiple costly and inefficient steps.
  • Significant product loss and increased costs are associated with conventional purification methods.

Purpose of the Study:

  • To develop an integrated cyclic process device for simultaneous clarification, concentration, and chromatographic purification of biomolecules.
  • To reduce the cost and product loss associated with biomolecule isolation from fermentation broths.

Main Methods:

  • A device integrating hollow-fiber ultrafiltration membranes with ion exchange chromatographic resin beads was designed.
  • Proteins were loaded onto the stationary phase via ultrafiltration permeate, followed by elution and collection of fractions.

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  • The process involved cyclic loading, elution, washing, and regeneration within a single unit.

    • Main Results:

    • Excellent resolution of binary protein mixtures (myoglobin, beta-lactoglobulin, hemoglobin, bovine serum albumin, alpha-lactalbumin) was achieved.
    • Successful purification of target proteins from a yeast-based cellular suspension was demonstrated.
    • The integrated device effectively performed clarification, concentration, and chromatographic purification.

    Conclusions:

    • The developed cyclic process device offers an efficient, integrated solution for biomolecule purification.
    • This technology is suitable for both extracellular and intracellular biomolecules, reducing processing steps and costs.
    • The device streamlines purification, minimizing product loss and enhancing overall process economics.