Related Experiment Video
Updated: Aug 11, 2026

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo
Published on: February 2, 2016
Distinct promoter elements mediate endodermal and mesodermal expression of the HNF1alpha promoter in transgenic
1Institut für Zellbiologie (Tumorforschung) Universitätsklinikum Essen, Hufelandstrasse 55, D-45122, Essen, Germany. gerhart.ryffel@uni-essen.de
Abstract:
The gene encoding the tissue specific transcription factor HNF1alpha is expressed in vertebrates in tissues of endodermal origin such as the liver and the gut as well as in the kidney, a mesoderm derived organ. Using a 6 kb HNF1alpha promoter fragment linked to GFP we observed green fluorescence in transgenic embryos restricted to the liver and gut as well as to the pronephros, the embryonic kidney. By deletion and mutation analysis of the HNF1alpha promoter we succeeded in dissecting the HNF1alpha promoter into two entities that are either active in the endoderm or the mesoderm. In conclusion, our data establish that the generation of transgenic Xenopus allows the functional dissection of promoters in the context of the entire organism.
Related Concept Videos
RNA Polymerase II Accessory Proteins
The Eukaryotic Promoter Region
Master Transcription Regulators
Reporter Genes
Commonly used reporter...

