A simple estimation method for the embryonic toxicity of plastic wrapping sheets using an egg shell-free culture...

To estimate the embryonic toxicity of food wrapping films, the shell-free chick embryos were cultured using plastic wrapping sheets like a suspending hammock. Polyethylene (PE) wrapping sheets with no additives showed much higher survivability than polyvinylchloride (PVC) wrapping sheets including plasticizers. Furthermore when a PVC wrapping sheet was autoclaved at 110 degrees C for 30 min, the embryonic survivability significantly decreased compared with the untreated PVC wrapping sheet. These observations suggest that the egg shell-free culture method using a plastic wrapping sheet is a useful estimation method for the embryonic toxicity of food wrapping film.