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Stem cell factor protects germ cells from apoptosis in vitro.
1Departments of Physiology and Pediatrics, University of Turku, Kiinamyllynkatu 10, Turku, Finland.
Journal of Cell Science
|December 11, 1999
Summary
Stem cell factor (SCF) protects adult rat germ cells, including spermatogonia, spermatocytes, and spermatids, from apoptosis. Follicle-stimulating hormone (FSH) also promotes germ cell survival partly via the SCF/c-kit pathway.
Area of Science:
- Reproductive Biology
- Cell Biology
- Endocrinology
Background:
- Stem cell factor (SCF) is crucial for germ cell development but its role in adult testes is unclear.
- Previous studies suggest SCF enhances spermatogonial survival and proliferation.
Purpose of the Study:
- To investigate the pro-survival function of SCF on adult rat germ cells in vitro.
- To determine if FSH-mediated germ cell survival involves the SCF/c-kit pathway.
Main Methods:
- Culturing adult rat seminiferous tubules with or without SCF and analyzing apoptosis via DNA laddering and ISEL staining.
- Blocking SCF/c-kit interaction with ACK-2 antibody to assess FSH's protective effect.
Main Results:
- SCF significantly reduced apoptosis in spermatogonia, spermatocytes, and spermatids.
- Apoptosis was delayed and less severe in SCF-treated tubules.
- Blocking SCF/c-kit partially abolished FSH's protective effect on germ cells.
Conclusions:
- SCF functions as a vital survival factor for adult rat germ cells.
- FSH's pro-survival effect on germ cells is partially mediated by the SCF/c-kit signaling pathway.