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TRIPLES: a database of gene function in Saccharomyces cerevisiae
A Kumar1, K H Cheung, P Ross-Macdonald
1Department of Molecular, Yale University, New Haven, CT 06520-8103, USA.
Nucleic Acids Research
|December 11, 1999
Summary
Researchers created a new database called TRIPLES to catalog yeast mutant strains. This resource provides data on phenotypes, localization, and expression, facilitating yeast research and reagent distribution.
Area of Science:
- Yeast genetics and genomics
- Bioinformatics and database development
- Molecular and cellular biology
Background:
- Transposon-based mutagenesis is a powerful tool for generating genetic diversity in Saccharomyces cerevisiae.
- Comprehensive databases are crucial for organizing and disseminating large-scale genetic and phenotypic data.
- Existing yeast databases may lack integrated information on transposon insertion phenotypes, localization, and expression.
Purpose of the Study:
- To develop a centralized, web-accessible database for cataloging a large collection of defined yeast mutant alleles.
- To provide a comprehensive resource for analyzing transposon insertion phenotypes, protein localization, and gene expression.
- To facilitate the distribution of valuable yeast mutant strains to the scientific community.
Main Methods:
- Generation of a collection of defined mutant alleles in Saccharomyces cerevisiae using a novel multipurpose mini-transposon.
- Development of the TRansposon-Insertion Phenotypes, Localization and Expression in Saccharomyces (TRIPLES) database.
- Integration of data including over 250,000 data points from nearly 7800 transposon-mutagenized yeast strains.
- Inclusion of external links to Saccharomyces Genome Database and GenBank, and online order forms for strain requests.
Main Results:
- The TRIPLES database provides access to data for over 2300 open reading frames.
- Detailed strain reports are available in table format or as downloadable tab-delimited text files.
- Users can directly request yeast strains free-of-charge via integrated online order forms.
Conclusions:
- TRIPLES serves as a unique and valuable resource for the Saccharomyces cerevisiae research community.
- The database enhances the analysis of gene function through comprehensive phenotype, localization, and expression data.
- TRIPLES promotes the efficient distribution of essential research reagents, accelerating scientific discovery.