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BodyMap: a human and mouse gene expression database.

T Hishiki1, S Kawamoto, S Morishita

  • 1Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.

Nucleic Acids Research
|December 11, 1999
PubMed
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BodyMap is a comprehensive gene expression database providing insights into human and mouse tissues. This resource enables researchers to analyze gene expression patterns and identify genes with specific expression profiles.

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • The BodyMap database has been curated since 1993, compiling extensive gene expression data.
  • It utilizes site-directed 3'-ESTs from non-biased cDNA libraries, encompassing over 270,000 sequences from 60 human and 38 mouse tissues.

Purpose of the Study:

  • To provide a robust resource for analyzing gene expression patterns across human and mouse tissues.
  • To enable in silico experiments for understanding mRNA composition and gene expression profiles.
  • To facilitate the selection of genes based on desired expression patterns in both species.

Main Methods:

  • Collection and curation of site-directed 3'-expressed sequence tags (ESTs) from non-biased cDNA libraries.
  • Grouping of ESTs to identify unique transcripts and quantify their abundance in different tissues.

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  • Cross-referencing with other public databases for data validation.

    • Main Results:

    • The database contains over 270,000 sequences from 60 human and 38 mouse tissues.
    • Site-directed ESTs allow for accurate transcript grouping and abundance estimation.
    • Enables three types of in silico experiments: mRNA composition estimation, gene expression pattern assessment, and gene selection based on expression profiles.

    Conclusions:

    • BodyMap serves as a valuable tool for researchers studying gene expression in humans and mice.
    • Its unique features support diverse in silico analyses, aiding in the discovery of genes with specific expression patterns.
    • The database is accessible online, promoting broader scientific research and collaboration.