Comparison of antigen binding capabilities of various membrane filters and filter papers in dot immunoassay

Antigen binding activities of 25 kinds of filter papers, including nitrocellulose (NC), nylon or polyvinylidine difluoride (PVDF), in the binding of 5 viruses, 3 bacteria, 2 mycoplasmas and 1 chicken serum protein antigens in dot immunoassay were compared. Immobilon affinity membrane type D (IAM-D) was found best in binding viral antigens, followed by Ultrabind SV-450 (SV-450). SV-450 was found best in binding bacterial antigen, followed by Ultrabind US-450 (US-450), IAM-D and NC 0.45 micron. IAM-D was the best in binding mycoplasma antigen, followed by Ultrabind HP, US-450, NC 0.2 micron. Overall, IAM-D had the best capability in the binding of the antigens.