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Updated: Aug 2, 2026
A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
Published on: July 6, 2009
A comparison of post-thaw results between cryopreserved embryos derived from intracytoplasmic sperm injection and
Y Hu1, W S Maxson, D I Hoffman
1Northwest Center for Infertility and Reproductive Endocrinology, Margate, Florida, USA.
Objective:
To study the effect of freezing on early stage embryos derived from intracytoplasmic sperm injection (ICSI) or from IVF.
Design:
Prospective, controlled clinical study.
Setting:
Private IVF center.
Patient(S):
Sixty-seven consecutive patients undergoing frozen-thawed embryo transfer cycles.
Intervention(S):
Early stage embryos were frozen, thawed, and transferred.
Main Outcome Measure(S):
Post-thaw survival, implantation and pregnancy rates.
Result(S):
We noted an 88% post-thaw survival rate, an 18% implantation rate, and a 52% pregnancy rate in the ICSI group and 81%, 11%, and 25%, respectively, with conventional fertilization.
Conclusion(S):
Early stage embryos (either zygote or 2-4 cells) derived from ICSI can be frozen with confidence and higher post-thaw survival and pregnancy rates can be achieved when compared with those from conventional IVF.
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