Related Experiment Video
Updated: Jul 31, 2026

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
Published on: August 30, 2018
Prevalent nosocomial gram negative aerobic bacilli and their antimicrobial susceptibility pattern in intensive care
1Department of Clinical Microbiology, Dr. Ziauddin Hospital, Karachi.
Objective:
To determine the type of prevalent aerobic gram-negative bacilli and their sensitivity pattern among nosocomial isolates.
Design:
Prospective collection of clinically significant nosocomial gram negative bacilli.
Setting:
Tertiary care hospital in Karachi.
Method:
One hundred isolates were identified by standard methods and minimum inhibitory concentration was checked by epsilometer test.
Results:
The most frequent isolates were Eschericia coli (43%) followed by Klebsiella pneumoniae (18%) Acinetobacter spp. (7%) Enterobacter spp. (7%) and Klebsiella spp. other than pneumoniae (7%). Most of the isolates of dominant species (E. coli and Klebsiella pneumoniae) were multiresistant including third generation cephalosporins.
Conclusion:
This study indicates that most effective antibiotics imipenem and amikacin inhibited most of the isolates. Imipenem alone or amikacin in combination with one broad spectrum beta-lactam drug should be used in initial empiric therapy in any life threatening nosocomial infection.
More Related Videos
08:04Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining
Published on: February 8, 2019
08:58Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
Related Concept Videos
Healthcare Associated Infections II: Preventive Measures
The best practices for preventing healthcare-associated infections include hand hygiene, patient risk...
Acute Pyelonephritis II: Diagnostic Studies and Management
Development of Antibiotic Resistance
Atypical Pneumonia
Mechanism of Antibiotic Resistance in MRSA
Clinical Significance of Antibiotic Resistance