Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Viability of deformed cells.

H Takamatsu1, B Rubinsky

  • 1Cooling Technology Laboratory, Institute of Advanced Material Study, Kyushu University, Kasuga, Fukuoka, 816-8580, Japan.

Cryobiology
|December 22, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Prognostic value of sequencing-based minimal residual disease detection in patients with multiple myeloma who underwent autologous stem-cell transplantation": [Annals of Oncology 28 (2017):2503-2510].

Annals of oncology : official journal of the European Society for Medical Oncology·2022
Same author

The pattern of cell survival in the pig liver following one freeze-thaw cryosurgery cycle.

Cryo letters·2022
Same author

Prostate cancer treatment with Irreversible Electroporation (IRE): Safety, efficacy and clinical experience in 471 treatments.

PloS one·2019
Same author

Time-dependent Effects of Pressure during Preservation of Rat Hearts in an Isochoric System at Subfreezing Temperatures.

Cryo letters·2019
Same author

Cryoelectrolysis for Treatment of Atrial Fibrillation: A First Order Feasibility Study.

Cryo letters·2018
Same author

The effect of isochoric freezing on mammalian cells in an extracellular phosphate buffered solution.

Cryobiology·2018

Cell damage during freezing can occur due to ice crystal compression, not just chemical changes. Human prostate cancer cells showed decreased viability when compressed, highlighting a new cryobiology insight.

Area of Science:

  • Cryobiology
  • Cell Biology
  • Biophysics

Background:

  • The prevailing theory attributes cryoinjury during slow freezing to extracellular solution hypertonicity.
  • Emerging evidence suggests mechanical stress, specifically cell compression by ice crystals, may also cause cell damage.

Purpose of the Study:

  • To investigate the impact of physical compression on cell viability during freezing.
  • To elucidate the role of ice crystal spacing and temperature in compression-induced cell damage.

Main Methods:

  • Developed a novel experimental setup to precisely control and apply compression to human prostate primary adenoma cancer cells during freezing.
  • Utilized microscopy to measure cell diameter reduction and assess cell viability post-compression.
  • Formulated a mathematical model correlating cell compression, ice crystal spacing, and temperature.

Related Experiment Videos

Main Results:

  • Cell viability significantly decreased when cells were compressed to 30% of their original diameter, indicating substantial mechanical stress.
  • This compression corresponds to a 50% increase in cell membrane surface area under the assumption of uniform membrane expansion.
  • The mathematical model, validated by experimental data, predicts compression-induced cell damage around -1.8°C when ice crystals are comparable in size to cells.

Conclusions:

  • Cell compression by ice crystals represents a significant mechanism of cryoinjury, independent of osmotic stress.
  • The temperature threshold for compression damage is dependent on ice crystal morphology and spacing.
  • Findings provide critical insights for optimizing cryopreservation protocols, particularly for sensitive cell types like cancer cells.