Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

EMAN: semiautomated software for high-resolution single-particle reconstructions.

S J Ludtke1, P R Baldwin, W Chiu

  • 1Verna and Marrs McLean Department of Biochemistry, National Center for Macromolecular Imaging, Houston, Texas 77030, USA.

Journal of Structural Biology
|December 22, 1999
PubMed
Summary

EMAN (Electron Micrograph ANalysis) is a new software package for single-particle reconstruction from electron micrographs. It enables high-resolution structural analysis beyond 10 Angstroms with automated contrast transfer function correction.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Baseline data for distribution of contaminants by natural disasters: results from a residential Houston neighborhood during Hurricane Harvey flooding.

Heliyon·2019
Same author

In reply 2.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2016
Same author

Single-Particle Refinement and Variability Analysis in EMAN2.1.

Methods in enzymology·2016
Same author

Tools for Model Building and Optimization into Near-Atomic Resolution Electron Cryo-Microscopy Density Maps.

Methods in enzymology·2016
Same author

Therapeutic drug monitoring of isoniazid and rifampicin during anti-tuberculosis treatment in Auckland, New Zealand.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2016
Same author

Huntington's disease cerebrospinal fluid seeds aggregation of mutant huntingtin.

Molecular psychiatry·2015

Area of Science:

  • Structural biology
  • Biophysics
  • Computational biology

Background:

  • Transmission electron microscopy (TEM) is crucial for determining molecular structures.
  • High-resolution single-particle reconstruction (SPR) requires sophisticated computational tools.
  • Existing software may lack comprehensive features for advanced SPR.

Purpose of the Study:

  • To introduce EMAN (Electron Micrograph ANalysis), a novel software package for semiautomated SPR.
  • To enable high-resolution reconstructions (beyond 10 Angstroms) from TEM data.
  • To provide a user-friendly yet powerful tool for researchers.

Main Methods:

  • Implementation of a complete SPR algorithm.
  • Iterative model refinement using classification and projection matching.

Related Experiment Videos

  • Simultaneous fitting of multiple micrographs for contrast transfer function (CTF) parameter determination.
  • Automated amplitude and phase CTF correction within the refinement loop.
  • Development of a graphical user interface (GUI) and shell command access.

    • Main Results:

    • EMAN provides options for initial model generation with varying symmetry (none, single-axis, icosahedral).
    • The software automates CTF parameter determination and correction.
    • Conformance tests with simulated data demonstrate the package's capabilities.
    • The package is written in C++ and freely available.

    Conclusions:

    • EMAN offers a comprehensive solution for semiautomated single-particle reconstruction.
    • Its advanced features, including automated CTF correction, facilitate high-resolution structural analysis.
    • The user-friendly interface and extensibility make it accessible to a wide range of researchers.