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PGE2 suppresses mitogen-induced Ca2+ mobilization in T cells
M A Choudhry1, P E Hockberger, M M Sayeed
1Trauma/Critical Care Research Laboratories, Departments of Surgery and Physiology, Burn & Shock Trauma Institute, Loyola University Chicago Medical Center, Maywood, Illinois 60153, USA.
Abstract:
PGE2-mediated suppression of T cell proliferation during sepsis could result from altered Ca2+ signaling. The present study evaluated the effects of PGE2 on Ca2+ release from intracellular stores and its influx through the plasma membrane in splenic T cells from Sprague-Dawley rats. Intracellular Ca2+ concentration ([Ca2+]i) responses in individual T cells were assessed using the Ca2+ imaging technique, and the release of Ca2+ from intracellular stores and Ca2+ influx were spectrofluorometrically quantified in T cell suspensions. Under unstimulated conditions, nearly 85% of T cells exhibited [Ca2+]i