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Related Experiment Videos

Age-related permeability changes in rabbit corneas.

T L Ke1, A F Clark, R W Gracy

  • 1Alcon Laboratories, Inc., Fort Worth, Texas 76134, USA.

Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics
|December 28, 1999
PubMed
Summary

Corneal drug penetration significantly decreases with aging in rabbits, impacting delivery efficiency. This age-related decline varies based on compound properties and corneal integrity.

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Area of Science:

  • Ophthalmology
  • Pharmacology
  • Biomedical Science

Background:

  • Corneal drug delivery is crucial for treating eye diseases.
  • Understanding age-related changes in corneal permeability is essential for optimizing drug efficacy.

Purpose of the Study:

  • To investigate the effect of aging on the corneal penetration of various compounds.
  • To determine how physicochemical properties and corneal integrity influence age-related differences in permeability.

Main Methods:

  • Utilized in vitro diffusion cells for passive transport under steady-state conditions.
  • Measured compound permeability across young (6 weeks) and old (3-4 years) rabbit corneas (intact and denuded).
  • Assessed a range of compounds with diverse lipophilicity and molecular weights.

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Main Results:

  • A significant decrease in corneal permeability was observed in aged corneas compared to young corneas.
  • The age-related difference in permeability was more pronounced for large hydrophilic compounds than small lipophilic ones in intact corneas.
  • The permeability difference between young and old denuded corneas was consistently around 2-fold.

Conclusions:

  • Aging significantly alters corneal permeability, affecting drug and nutrient transport.
  • Fundamental biochemical differences exist between young and aged corneas.
  • Findings provide insights for developing improved strategies for corneal drug and nutrient delivery.